Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity.
Promega ADP-Glo™ Kinase Assay + MINK1 Kinase Enzyme System
Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes.
Promega P450-Glo CYP2B6 Assay, 50ml
The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega ADP-Glo™ Kinase Assay + ERK2 Kinase Enzyme System
Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + PAK4 Kinase Enzyme System
Recombinant full-length human PAK4 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. PAK4 is a member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility and transformation.
Promega P450-Glo CYP2C19 Screening System, 1,000 assays
The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega P450-Glo™ CYP3A4 Assay (Luciferin-PPXE) DMSO-Tolerant Assay
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
Promega P450-Glo™ CYP3A4 Assay with Luciferin-IPA
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
Promega P450-Glo™ CYP1A2 Induction/Inhibition Assay
The P450-Glo™ CYP1A2 Assay and Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega GSH-Glo™ Glutathione Assay
The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples.
Promega NAD(P)H-Glo™ Detection System
The NAD(P)H-Glo™ Detection System is a bioluminescent assay that measures the concentration of the reduced forms of nicotinamide adenine dinucleotides, NADH and NADPH, and does not discriminate between them.
Promega ONE-Glo™ Assay
The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health. The assay uses a two-step, addition-only process to measure in a single well of a plate.
Promega ADP-Glo™ Kinase Assay + MLK1 Kinase Enzyme System
Recombinant human mLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
Promega WNK1 Kinase Enzyme System, 1mg
Recombinant human WNK1 (181-507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + PKCθ Kinase Enzyme System
Recombinant full-length human PKC theta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C theta (PKC theta) is an important component in the intracellular signaling cascade.